RILs are developed by:
Recombinant Inbred Lines are immortal homozygous mapping population developed by repeatedly selfing progeny from single cross through single seed descent without selection for six to eight generations. Starting from F2, one seed per plant is advanced randomly; heterozygosity halves each generation, approaching 99% homozygosity by F6, genotype fixed as mixture of parental alleles in homozygous form through recombination. Each RIL represents unique mosaic of parental chromosomes after multiple recombination events, capturing increased mapping resolution compared to F2 due to accumulated crossove
Ref: Burr & Burr 1991 RIL development; Singh & Singh Plant Breeding – SSD repeated selfing method
