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#molecular methods

2 public questions tagged with this topic.

BOTANY

Which of the following statements about molecular taxonomy is correct?

Molecular taxonomy classifies organisms by comparing DNA, RNA, and protein sequences, revealing evolutionary relationships. This follows from latest NCERT 2026-27 principle explaining the concept clearly for NEET students in simple steps as per rationalized syllabus.

Ref: NCERT Biology Textbook - Latest Edition for Academic Session 2026-27 (Botany section, Rationalized Textbook for Class XI and XII), Chapter: Biology - Botany portion (Latest NCERT Textbooks for Academic Session 2026-27 -

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Molecular Marker

A researcher chooses a method that is simple and fast for TMV resistance detection. Which is best?

Random amplified polymorphic DNA offers rapid, inexpensive screening without requirement for sequence information, radioactive probes or sophisticated instrumentation. Using a single short random primer in a standard PCR, numerous loci are amplified simultaneously, producing easily scorable fingerprints on agarose gels within hours. For preliminary disease resistance screening such as Tobacco mosaic virus, quick identification of linked fragments is advantageous. RFLP and AFLP involve laborious digestion, blotting and selective amplification steps, while EST-SSR development requires transcript

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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