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#Marker-Assisted Backcrossing

2 public questions tagged with this topic.

Plant Breeding 4

Background selection in MABC helps in:

Background selection accelerates recovery of recurrent parent genome beyond theoretical expectation using genome-wide molecular markers distributed across all chromosomes. While foreground selection tracks donor gene, background selection scans chromosomes unlinked to target using polymorphic SSR or SNP markers distinguishing recurrent and donor alleles by size or sequence. In each backcross generation, individuals carrying target gene are genotyped across background, and those with highest proportion of recurrent parent alleles are chosen as parents for next backcross. Conventional backcrossi

Ref: Frisch M et al. 1999 Crop Sci – background selection for RPG recovery; Ribaut JM MAS strategies

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Plant Breeding 4

Foreground selection in MABC focuses on:

Marker-assisted backcrossing comprises two selection layers that accelerate recovery and ensure gene retention. Foreground selection focuses directly on target locus introgressed from donor, using gene-specific or tightly linked markers less than 2 cM to ensure presence of desired allele in each backcross generation. In early generations BC1F1, BC2F1, individual seedlings genotyped for foreground markers, carriers retained, non-carriers discarded, avoiding waiting for phenotypic expression that may require disease inoculation or recessive homozygosity assessment. This prevents accidental loss

Ref: Hospital F & Charcosset A 1997 Genetics – MABC foreground selection. Collard & Mackill marker review

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