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Oil left hardens, attracts dust, damages lens coating and can seep into objective. Clean with lens paper and xylene-free cleaner after each session, never use tissue.
Ref: NCERT Biology Laboratory Manual, Class XI, Mounting precautions and identification of artifacts.
Without oil, light refracts from glass to air (n=1.0) and is lost by total internal reflection. Oil bridges gap, allowing higher NA and resolution.
Ref: Nikon MicroscopyU, Parfocality and field loss during objective switching, troubleshooting.
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