Cancer gene therapy often uses strategy of:
Cancer gene therapy predominantly adopts tumoricidal strategy rather than restoration tumor suppressor because cancer genome carries multiple gain-of-function oncogenes KRAS MYC aneuploidy heterogeneity complicating simple addition. Methods eliminate malignant clone through introduction genes triggering death or immune recruitment. Oncolytic adenoviruses engineered deletion E1B 55 kDa protein normally inactivating p53 replicate selectively p53-deficient cancers lysing cells via viral burst releasing PAMPs tumor antigens danger signals ATP calreticulin HMGB1 driving immunogenic cell death. Suic
Ref: Nature Reviews Cancer Gene Therapy Oncolytic Mechanisms 2021; NCI Cancer Gene Therapy Targeted Killing; Molecular Therapy Oncolytic Viruses 2021.
