Who developed flow cytometry?
Flow cytometry evolved from principles of Coulter volume sensing and fluorescence detection of single cells in fluid stream. Mack Fulwyler in 1965 described the first fluorescence based cell sorter separating cells by volume, publishing electrostatic deflection principle that became foundation of modern instruments at Los Alamos. Engvall and Perlmann developed ELISA methodology, Rosalyn Yalow pioneered radioimmunoassay for insulin measurement. Fulwyler innovation enabled hydrodynamic focusing, laser interrogation and charged droplet sorting, allowing quantitative analysis of size, granularity
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
