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Plant tissue culture 2

Cells that inherently possess embryogenic potential are called:

Cells inherently possessing embryogenic potential without need for prolonged dedifferentiation are designated pre embryogenic determined cells PEDCs conceptualized by Sharp et al. They exist in tissues retaining embryonic character such as nucellus, immature zygotic embryo protoderm, shoot apical meristem periphery and leaf epidermis of certain species. Epigenetically PEDCs maintain open chromatin at promoters of master regulators SOMATIC EMBRYOGENESIS RECEPTOR KINASE1 SERK1 BABY BOOM BBM LEAFY COTYLEDON1 LEC1 and WUSCHEL, with low DNA methylation allowing rapid transcriptional activation. Upo

Ref: Sharp et al., 1980 PEDCs concept; Ikeuchi et al., Development 2013 cellular reprogramming.

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Plant tissue culture 1

Shoot tip culture is an example of:

Shoot tip culture qualifies as organ culture because explant retains organized structure of shoot apical meristem plus one to three leaf primordia and continues organized growth maintaining polarity and tissue layer arrangement tunica corpus. Unlike callus culture where organization is lost into unorganized parenchyma, or cell suspension where aggregates disperse in liquid agitation, or protoplast culture where wall removed exposing naked membrane, shoot tip preserves defined zonation central zone peripheral zone rib meristem and procambium differentiation pathway. Culture on hormone balanced

Ref: George et al., Plant Propagation Tissue Culture Vol1; Hartmann organ culture.

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Plant tissue culture 1

Surface sterilization of explants commonly uses:

Surface sterilization removes epiphytic and surface-borne microorganisms from explants without killing plant tissues, essential prerequisite for establishing contaminant-free cultures. Most effective protocol uses sequential treatment with 70 percent ethanol for 30-60 seconds followed by sodium hypochlorite solution 0.5-2 percent available chlorine for 10-20 minutes containing surfactant Tween-20 0.1 percent to improve wetting. Ethanol acts as degreasing agent dissolving epicuticular waxes, disrupting microbial lipid membranes and denaturing proteins facilitating penetration of subsequent ster

Ref: Bhojwani & Dantu Plant Tissue Culture, Chap. 3 sterilization; NCBI StatPearls Aseptic Technique.

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