The Michaelis-Menten constant (Km) is numerically equal to:
The substrate concentration at V = 0.5 Vmax is obtained by applying the relevant formula or quantitative relationship to the given parameters. In Enzyme Kinetics, numerical problem-solving requires understanding the mathematical relationships between biological variables. The calculation involves substituting the provided values into the appropriate equation and solving systematically. The other options (Half of Vmax, The concentration of enzyme in the reaction, and The total product formed in the reaction) result from common calculation errors such as using incorrect formulas, misidentifying
Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 6
