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#drug testing

2 public questions tagged with this topic.

Animal Tissue Culture 3

The phase of cell growth most suitable for drug testing is:

Pharmacological testing and chemosensitivity evaluation are most predictive during logarithmic exponential growth phase when cell population is actively cycling, metabolically robust, and homogeneous. During lag, cells are still recovering and fraction in S phase low, causing underestimation of antimetabolite efficacy. In log phase, DNA synthesis enzymes including topoisomerase II, thymidylate synthase, and kinases targeted by many drugs are highly expressed, mitochondrial activity high, ATP levels stable. Dose-response curves generated in this window show reproducible IC50 values. Plateau or

Ref: Freshney Ch.21 Drug testing log phase; NIH Assay Guidance Manual 2021 Chemosensitivity assays log phase optimal viability.

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Animal Tissue Culture 3

The in vitro system closest to in vivo liver toxicity testing is:

Cultured animal cells divide into two fundamental categories with distinct biological properties. Primary cultures derived directly from tissue explants retain finite lifespan, diploid karyotype, anchorage dependence, contact inhibition, and tissue-specific functions such as albumin secretion by hepatocytes, but undergo senescence after limited doublings due to telomere shortening and activation of p53-p16 checkpoints. Continuous or established lines arise via spontaneous or experimentally induced transformation events that inactivate tumor suppressors, activate telomerase hTERT maintaining te

Ref: Freshney Ch.15 Primary vs continuous cell lines finite vs infinite; Alberts MBoC Ch.20 Transformation and immortalization characteristics.

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