The site of the division plane during cytokinesis in animal cells is determined by:
Cytokinesis in animal cells determines placement of furrow such that daughter cells inherit single genome copy and appropriate cytoplasmic volume. Experimental embryology demonstrated furrow forms where central spindle contacts cortex. Mechanistically, anaphase central spindle assembled from overlapping antiparallel interpolar microtubules bundled by PRC1 and centralspindlin component KIF4 recruits centralspindlin heterotetramer consisting of kinesin MKLP1 and RhoGAP MgcRacGAP. Centralspindlin clusters ECT2 RhoGEF, generating zone of active RhoA-GTP precisely at equatorial membrane. RhoA-GTP a
Ref: Fededa & Gerlich, Current Biology 2012, Division Plane Specification. Alberts 7th ed., Chapter 17.
