Which factor is required for dissociation of the SNARE complex?
After fusion membrane-embedded SNARE complex ends up as cis-complex where all helices reside in same membrane representing dead-end product incapable of further fusion blocking availability. To maintain flux complex must be disassembled into monomers. Alpha-SNAP adaptor family including alpha, beta, gamma isoforms binds along four-helix bundle recognizing charge pattern; up to four molecules coat complex serving landing pad for hexameric NSF ATPase. NSF contains N-terminal substrate binding domains, D1 ATPase providing mechanical power and D2 stabilizing hexamer. ATP hydrolysis in D1 hydrolyzi
Ref: Alberts et al., MBC: NSF ATPase disassembles SNARE complexes post-fusion for recycling.
