What type of bond formation occurs in myristoylation?
Myristoylation represents cotranslational irreversible lipidation catalyzed by cytosolic N-myristoyltransferases NMT1 and NMT2. After methionine aminopeptidase removes initiator methionine, glycine at position two becomes exposed as acceptor. NMT binds myristoyl-CoA, a fourteen-carbon saturated fatty acyl thioester, and transfers acyl chain to alpha-amino group of N-terminal glycine forming stable amide linkage resistant to neutral hydroxylamine, alkaline treatment, and reducing agents, unlike labile thioesters. The myristate provides approximately eight kilocalories per mol hydrophobic insert
Ref: Lodish et al., Molecular Cell Biology, 9th ed., Chapter 10, Protein Lipidation and Myristoylation.
