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#cortical rotation

2 public questions tagged with this topic.

amphibian Development-l

Cortical rotation in frog eggs involves rotation of:

Cortical rotation in frog eggs involves coordinated movement of outer cortical cytoplasm relative to inner endoplasm. After fertilization-induced calcium wave, subcortical microtubules polymerize parallel to future dorsal-ventral axis and kinesin motors transport cortex containing membrane-anchored Dishevelled, Wnt11 mRNA, and GBP about thirty degrees toward side opposite sperm entry. Central yolk cytoplasm remains stationary, creating shear zone. This repositioning brings beta-catenin stabilizing factors dorsally where GSK-three inhibition occurs, allowing nuclear accumulation and formation o

Ref: Wolpert, Principles of Development, 6th ed., Chapter 3: Cortical rotation outer cortex movement.

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frog development

Which molecule accumulates dorsally during cortical rotation?

Cortical rotation generates aligned microtubule tracks between vegetal pole and future dorsal side allowing kinesin-directed transport of membrane-associated proteins and mRNAs. Critical cargo includes Dishevelled protein, Wnt11 mRNA and glycogen synthase kinase binding protein that inhibits GSK3 locally. Accumulation dorsally results in local stabilization of beta-catenin which then enters nuclei of dorsal vegetal and marginal blastomeres forming Nieuwkoop center. BMP and noggin not transported dorsally in this cytoskeletal event, FGF more uniform. Therefore molecule accumulating dorsally dur

Ref: Moon and Kimelman, Wnt signaling and cortical rotation, Developmental Biology Review, Beta-catenin asymmetry.

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