Simple sequence repeats represent tandem repeats of one to six base pair motifs dispersed throughout eukaryotic genomes. They exhibit codominance, allowing clear distinction between homozygous and heterozygous states based on fragment length polymorphism. Each allele is amplified with locus-specific primers flanking the repeat, producing different sized products. In contrast, RAPD, AFLP and ISSR behave as dominant systems scoring only presence or absence of bands. High reproducibility, multiallelism and Mendelian inheritance make SSR ideal for mapping, diversity analysis and marker-assisted se
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NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.