BHK21 cells are commonly employed in:
Plateau phase, also termed stationary phase, occurs when culture reaches carrying capacity constrained by surface area for adherent cells or nutrient limits for suspension cells. Cell number stabilizes as division rate equals death rate, curve flattening. Molecular mechanisms include contact inhibition mediated by Hippo pathway: high cell density activates LATS1/2 kinases phosphorylating YAP leading to cytoplasmic retention and degradation preventing transcription of cyclin D1 and survivin. Cadherin engagement also induces p27Kip1. Simultaneously growth factor exhaustion, accumulation of inhib
Ref: Alberts MBoC Ch.17 Plateau contact inhibition Hippo YAP; Freshney Ch.13 Growth curve plateau stationary quiescence balance.
