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#bacterial stress

2 public questions tagged with this topic.

Microbial Physiology

The heat shock response in bacteria involves:

Sudden increase in ambient temperature from 30 to 42 degrees Celsius and beyond causes widespread protein unfolding, misfolding and aggregation, exposing normally buried hydrophobic patches that drive non-native interactions and toxic oligomerization threatening proteome integrity. Bacterial heat shock response is orchestrated by alternative sigma factor sigma32 encoded by rpoH gene, whose expression is regulated at multiple levels including translation efficiency enhanced at high temperature via melting of inhibitory mRNA secondary structure and protein stability controlled by DnaK-DnaJ chape

Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 7: Heat Shock Response and Chaperone Upregulation.

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Microbial Physiology

The primary function of glutathione in bacterial stress response is:

Glutathione is a low-molecular-weight tripeptide gamma-glutamyl-cysteinyl-glycine present at millimolar concentrations up to 10 mM in many Gram-negative bacteria such as Escherichia coli and few Gram-positives that synthesize it via GshA and GshB. Reduced form GSH serves as major thiol buffer and electron donor protecting against reactive oxygen species generated continuously by endogenous respiratory electron transport and by host immune oxidative burst involving NADPH oxidase-dependent production of superoxide during phagocytosis. It directly scavenges superoxide anion, hydroxyl radical and

Ref: Prescott's Microbiology, 11th ed., Chapter 7: Glutathione and Oxidative Stress Protection in Bacteria.

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