Which inhibitor blocks autophagosome-lysosome fusion?
Autophagic flux progresses through initiation, elongation, closure, fusion and degradation. Efficient cargo clearance requires autophagosome lysosome fusion mediated by small GTPase RAB7, HOPS complex and SNARE proteins STX17 SNAP29 VAMP8 and YKT6, plus tethering factors like PLEKHM1. Bafilomycin A1, a macrolide isolated from Streptomyces griseus, is a high affinity inhibitor of vacuolar type H+ ATPase V0 domain c subunit, blocking proton pumping into lysosome lumen. Collapse of acidic pH inactivates cathepsins and prevents LysoTracker accumulation. Recent work shows additional target ER calci
Ref: NCBI Bookshelf, Molecular Biology of the Cell, Section: Autophagosome-Lysosome Fusion and V-ATPase Inhibitors.
