The slope of a Lineweaver-Burk plot is equal to:
Km/Vmax is obtained by applying the relevant formula or quantitative relationship to the given parameters. In Km and Vmax calculation, numerical problem-solving requires understanding the mathematical relationships between biological variables. The calculation involves substituting the provided values into the appropriate equation and solving systematically. The other options (Vmax/Km, -Km/Vmax, and 1/Vmax) result from common calculation errors such as using incorrect formulas, misidentifying variables, inverting ratios, or making arithmetic mistakes.
Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 6