Skip to content

#meristem culture

6 public questions tagged with this topic.

Meristematic tissues are virus free because:

Meristematic tissues remain virus free because virus multiplication and movement are inhibited in rapidly dividing apical dome cells. Most plant viruses rely on plasmodesmata intercellular connections and phloem sieve tubes for systemic translocation from source leaves to sinks; meristem dome lacks differentiated phloem and contains narrow plasmodesmata with high callose deposition limiting viral cell to cell trafficking. Additionally mitotic cycle of meristem cells is significantly shorter than time required for virus replication and assembly, so daughter cells produced outrun infection front resulting in exclusion. High metabolic rate leads to elevated endogenous auxin levels and high oxygen radical scavenging antioxidant activity creating unfavorable environment for viral polymerase function. Molecular evidence indicates strong RNA silencing response in meristem with high expression of Dicer like proteins DCL1-4 and RNA dependent RNA polymerase RDR6 generating small interfering RNAs that degrade viral genomes. Consequently even severely infected mother plant harbors distal 0.1 millimeter virus free zone exploitable for phytosanitation, providing basis for meristem culture combined with heat therapy for eradication of persistent viruses in vegetatively propagated crops.

Ref: Cassells 1991 virus free meristem; Lodish Chap. 8 viral movement.

Meristem culture is mainly used to produce:

Meristem culture technique involves aseptic excision of apical dome typically 0.1-0.5 millimeter containing tunica corpus but no differentiated leaf primordia and culture on MS based medium for regenerating plants principally to obtain virus free stocks. Foundation lies in observation that meristematic dome lacks differentiated vascular sieve elements through which many systemic viruses like Potato Virus Y, Tobacco Mosaic Virus, Cucumber Mosaic Virus travel long distance and also that rapid mitotic activity outpaces viral replication. High endogenous auxin and cytokinin concentration plus RNA silencing machinery Dicer like DCL proteins Argonaute AGO targeting viral RNAs creates antiviral niche. Protocol often combined with thermotherapy incubating donor at 35-38 degrees for weeks reducing virus titer or chemotherapy with antiviral ribavirin. Regenerants are indexed by ELISA RT PCR to confirm elimination. Because meristem cells maintain genetic stability avoiding callus mediated somaclonal variation, meristem derived plants retain true to type phenotype valuable for certified seed programs for potato garlic banana strawberry where vegetative propagation accumulates viruses causing yield decline, thus meristem culture ensures healthy foundation material.

Ref: Bhojwani & Dantu Chap. 11 virus elimination; Walkey 1978 Phytopath Z.