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Question

Which of the four restriction enzymes given below cut the following DNA sequence? 5’-CCGATATCTCGAGGGC-3’ (P) BamH1 (3’-CCTAG^G-5’) (Q) XhoI (3’-GAGCT^C-5’) (R) EcoRI (3’-CTTAA^G-5’) (S) EcoRV (3’-CTA^TAG-5’)

Options

Choose one · Correct answer highlighted

Explanation

Mechanistically, enzyme kinetics involves substrate binding and catalytic efficiency determine reaction rates. This validates Q & S because it reflects active-site specificity and regulation, a pattern repeatedly demonstrated in biochemistry research.

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