Practice question

MCQ

Question

Select the correct primer pair of forward and reverse primer sequence that amplify full length of sequence given below : 5′ – TAC TAT CGA GTA CTA GAC TAC TAAGT 1 – 100 – 1 ACT, AGA, CGT ACG, TAG, TAC, GTT ACT AC – 3′

Options

Choose one · Correct answer highlighted

5′ – ATG ATA GCT CAT GAT CTG AT – 3′ Forward 5′ – GTA GTA ACG TAC TAC GTA CG – 3′ Reverse 5′ – TAC TAT CGA GTA CTA GAC TA – 3′ Forward 5′ – GTA GTA ACG TAC TAC GTA CG – 3′ Reverse Correct 5′ – ATG ATA GCT CAT GAT CTG AT – 3′ Forward 5′ – GCA TGC ATC ATG CAA TGA TG – 3′ Reverse 5′ – TAG TCT AGT ACT CGA TAG TA – 3′ Forward 5′ – GCA TGC ATC ATG CAA TGA TG – 3′ Reverse

Explanation

Forward primer must match 5' end of coding strand to amplify forward direction, while reverse primer must be reverse complement of 3' end to anneal opposite strand. Option B provides forward identical to 5' sequence and reverse reverse-complementary to 3' sequence, enabling full-length amplification.

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Genetic 25 Sep 2026

Practice question

Select the correct primer pair of forward and reverse primer sequence that amplify full length of sequence given below : 5′ – TAC TAT CGA GTA CTA GAC TAC TAAGT 1 – 100 – 1 ACT, AGA, CGT ACG, TAG, TAC, GTT ACT AC – 3′

Options

Explanation

Comments

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