Practice question

Reverse-phase protein microarrays represent an inverted configuration compared to conventional forward arrays. Instead of printing capturing agents, complex biological samples such as tissue lysates, cell extracts, or body fluids are directly spotted onto slide, with each spot representing entire proteome of one sample. Subsequent detection uses specific antibodies against target protein or modified residue, enabling quantification of protein expression or phosphorylation across many clinical samples simultaneously. This format does not print antibodies as capture, does not rely on whole cells alone, and does not require TEV protease, which belongs to TAP methodology.