Practice question

MCQ

Question

Match the List I with List II. List I List II (A) Taq DNA polymerase (I) used to make cDNA (B) MMLV Reverse Transcriptase (II) used to phosphorylate oligos or DNA (C) RNA polymerase II (III) used to amplify DNA (D) T₄ poly nucleotide kinase (IV) Transcription particularly of mRNA, Ln cRNA

Options

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(A)-(IV), (B)-(III), (C)-(I), (D)-(II) (A)-(III), (B)-(IV), (C)-(I), (D)-(II) (A)-(I), (B)-(II), (C)-(III), (D)-(IV) (A)-(III), (B)-(I), (C)-(IV), (D)-(II) Correct

Explanation

Taq polymerase thermostable enzyme drives PCR amplification. MMLV reverse transcriptase synthesizes cDNA from mRNA templates. RNA polymerase II transcribes mRNA and lncRNA in eukaryotes. T4 polynucleotide kinase transfers phosphate to 5' ends of DNA oligos for labeling or ligation.

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