Practice question
Question
For ELISPOT measurements of interferon g [IFN-g] secretion by mouse NKT cells, the following steps were performed in an ELISA plate: A. Addition of purified spleenocytes B. Stimulation of spleenocytes by peptide pulsed antigen presenting cells (APCs) C. Addition of biotin-conjugated anti IFN-g antibody D. Addition of capture antibody specific to IFN-g E. Addition of avidin-conjugated colour changing substrate. Which one of the following options lists the correct sequence of steps performed?
Explanation
Analysis of for elispot measurements of interferon g [ifn-g] shows D→ A→ B→C→E represents established outcome. functional assays supports option C, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.
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