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Question

E. coli is grown exclusively in a medium containing ¹⁵ NH ₄ Cl as the sole nitrogen source. Subsequently, the cells were shifted to a medium containing ¹⁴ NH ₄ Cl. The molar ratio of hybrid DNA ( ¹⁵ N ¹⁴ N) to light DNA ( ¹⁴ N ¹⁴ N) after four generations (rounded off to two decimal places) will be _____ . (NAT)

Explanation

DNA replication fidelity analysis shows polymerase proofreading and origin firing ensure accurate duplication. Consequently Option NAT emerges as the valid choice since it matches high-fidelity synthesis mechanisms, aligning with established principles in molecular biology literature.

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