Practice question

MCQ

Question

Dpnl is used to digest the PCR product during site directed mutagenesis because

Options

Choose one · Correct answer highlighted

Dpnl digests irrespective of methylation status of DNA Dpnl digests only unmethylated DNA Dpnl digests only methylated DNA Correct Dpnl digests GC-rich sequences

Explanation

Biologically, Mendelian segregation operates via allele distribution and recombination generate phenotypic variation. Thus Dpnl digests only methylated DNA is appropriate since it follows segregation and linkage principles, reinforcing core concepts taught in genetics.

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