Practice question

MCQ

Question

Depth of field decreases with:

Options

Choose one · Correct answer highlighted

Higher NA and higher magnification, requiring fine focus to see different planes Correct Lower magnification Closing condenser Using mirror

Explanation

DOF ∝ λ / NA². Oil immersion 1.4 NA has DOF ~0.2µm vs 4x 0.1 NA DOF ~50µm. So bacteria at different depths need refocus.

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