Practice question
Question
A green fluorescent protein (GFP) encoding gene is fused to a gene encoding specific protein for expression in cells. What is the advantage of using GFP over staining cells with fluorescently labeled antibodies that bind to the target protein?
Explanation
In biochemistry, enzyme kinetics dictates that substrate binding and catalytic efficiency determine reaction rates. Therefore Its fusion allows tracking the location of the protein in living cells, while... is correct as it reflects active-site specificity and regulation, supported by mechanistic studies and conserved across related systems.
Discussion
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