Practice question

Eukaryotes lack a direct RRF ortholog and utilize alternative surveillance and recycling machinery with different protein composition. Pelota, also called Dom34 in yeast, structurally resembles eRF1 and forms a complex with GTPase Hbs1, a relative of eRF3 and EF-Tu that recognizes stalled ribosomes and empty A-sites. Dom34-Hbs1 together with the ABC ATPase ABCE1, also called Rli1 in yeast, drives splitting of 80S into 40S and 60S subunits ATP-dependently. This pathway also participates in no-go decay and nonstop decay, clearing aberrant messages, differing mechanistically from bacterial RRF-EF-G-IF3 mediated recycling architecture that requires tRNA mimic dynamics.