Practice question

Peptidyl-tRNA carrying nascent polypeptide attached via ester bond to 3' CCA adenosine resides predominantly in P site during elongation stage between translocation events. After peptide bond formation peptidyl chain initially on A-site tRNA, EF-G driven translocation moves tRNA-mRNA complex, placing deacylated tRNA in E site and peptidyl-tRNA in P site. P-site interaction stabilizes reading frame via codon-anticodon plus ribosomal protein contacts, and before termination its ester bond is substrate for release factor-mediated hydrolysis freeing completed protein after stop codon recognition.