Practice question

Peptide linkage forms via aminolysis reaction between alpha-amino group of A-site aminoacyl-tRNA and carbonyl carbon of ester-linked peptidyl-tRNA in P-site. Specifically, nucleophilic NH2 of incoming amino acid attacks electrophilic C=O carbon of peptide attached to 3' terminal adenosine of P-site tRNA CCA end, creating new amide bond between C-terminus of growing chain and N-terminus of incoming amino acid, transferring chain to A-site tRNA. Reaction driven by 23S rRNA positioning and exclusion of water, requiring no additional energy input beyond peptide ester hydrolysis.