Practice question

Partial digestion is defined by incomplete hydrolysis across the population of DNA molecules, usually caused by conditions that lower enzyme efficiency rather than increase it. Sub-optimal temperature, improper pH, suboptimal Mg2+ or salt concentration, presence of inhibitors like EDTA, SDS, residual organic solvents, short incubation time, or insufficient enzyme quantity prevent every site from being cut. Fully saturated enzymes and random cleaving suggest excessive or nonspecific activity. Blunt versus sticky nature of enzyme does not cause partiality, only reaction environment does.