Practice question

Okazaki fragments, 1000 to 2000 nucleotides long in bacteria and 100 to 250 in eukaryotes, are short nascent DNA segments observed during brief pulse labeling experiments. They appear exclusively on lagging strand because its template synthesized opposite to fork movement. Each fragment begins with 10 to 12 nucleotide RNA primer made by primase that provides essential 3' OH for Pol III extension. Polymerase extends until reaching previous fragment then displaces or awaits processing. Leading strand shows no such fragments under normal physiological conditions.