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Dmitri Ivanowsky observed that the agent causing tobacco mosaic disease passed through bacteria-proof filters. This indicated that the infectious agent was smaller than bacteria.
Ref: NCERT Class 11 Biology Chapter 2: Biological Classification Bacteria - Structure and Economic Importance
The history of virology is detailed in the NCERT text. It specifically credits 'D.J. Ivanowsky (1892)' with the formal recognition of certain microscopic infectious agents as the causal organisms of tobacco mosaic disease. He astutely observed that these agents were capable of passing through standard bacteria-proof filters, conclusively proving that they were significantly smaller than conventional bacteria.
Ref: NCERT Class 11 Biology > Chapter 2: Biological Classification > 2.6 Viruses, Viroids, Prions and Lichens
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