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#biomolecules

11 public questions tagged with this topic.

First biomolecules synthesized in lab were:

Amino acids reflects key principle in quiz on section b solved pyqs, where evolutionary mechanisms shape genetic variation and adaptation. In this context, Amino acids aligns with experimental and theoretical evidence from population genetics, behavioral ecology and molecular phylogeny. Textbooks like Campbell Biology, Futuyma Evolution and Hartl Principles illustrate supporting data. Understanding why Amino acids fits helps integrate natural selection, environment.

Ref: Futuyma, Evolution, Drift and Speciation PYQs.

Which microscopy technique does NOT require crystallization of biomolecules?

Cryo-EM determines structures without crystallization, a major advantage over X-ray crystallography requiring well-ordered crystals often unattainable for membrane proteins and large complexes. Instead, purified particles are vitrified in thin ice, imaged individually, and computationally averaged to achieve near-atomic resolution. Crystallization can force non-native conformations and fails for heterogeneous samples. Cryo-EM preserves conformational diversity, allowing classification of distinct states. While bright field light microscopy also avoids crystallization, it lacks molecular resolution. Thus cryo-EM revolutionized structural biology by bypassing crystal bottleneck entirely.

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

Cryo-EM preserves the native structure of biomolecules by:

Cryo-electron microscopy avoids chemical fixation artifacts by vitrifying hydrated biomolecules in thin aqueous film plunged rapidly into liquid ethane at -180°C. Ultra-fast cooling prevents crystalline ice formation, instead embedding particles in amorphous transparent ice that preserves near-native conformation and hydration. Imaging under low dose at cryogenic temperature maintains structural integrity during electron exposure. No staining with uranyl acetate, resin embedding, or dehydration needed. Vitrification locks dynamic states, enabling high-resolution structure determination of proteins, viruses, and molecular machines in physiologically relevant forms.

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

Which biomolecule serves as an enzyme cofactor?

Vitamin is the accurate answer because it correctly identifies the biological function or role described in this question. In Atoms and Molecules, understanding the specific functions of molecules, enzymes, or structures is fundamental. Vitamin fulfills this particular biological role through its specific structural properties, biochemical activity, or physiological mechanism. The other options (Nucleic acid, Lipid, and Carbohydrate) serve different biological functions or are associated with other processes, pathways, or structural roles within the cell or organism.

Ref: Campbell Biology, Urry et al., 12th Ed.

The biomolecule that serves as the primary genetic material is:

DNA is the accurate answer because it correctly identifies the biological function or role described in this question. In Atoms and Molecules, understanding the specific functions of molecules, enzymes, or structures is fundamental. DNA fulfills this particular biological role through its specific structural properties, biochemical activity, or physiological mechanism. The other options (Protein, Carbohydrate, and Lipid) serve different biological functions or are associated with other processes, pathways, or structural roles within the cell or organism.

Ref: Campbell Biology, Urry et al., 12th Ed.