Practice question

Ninety-six well microtiter plate manufactured from treated polystyrene provides optimal format for enzyme-linked immunosorbent assays. Wells accommodate 100-300 microliter volumes, allow uniform coating of antigen or antibody through passive adsorption and ensure consistent path length for spectrophotometric reading at 450 nm or 405 nm. Standardized footprint fits automated washers, multichannel pipettes and plate readers, enabling high throughput screening of up to 96 samples including standards and controls. Smaller volumes in 384-well plates reduce sensitivity, while 6-well or 12-well formats waste reagents and lack ELISA reader compatibility.