Practice question

Random amplified polymorphic DNA utilizes short decamer primers of arbitrary sequence to amplify anonymous loci across the genome under low stringency PCR conditions. Because primers are random, no reference genome or prior sequence information is required, enabling rapid screening in non-model species. Polymorphism arises from nucleotide changes at priming sites altering amplification. In contrast, SSR development needs flanking sequence for primer design, RFLP requires probes and restriction maps, and SNP discovery depends on sequencing data to identify single nucleotide variations.