Practice question

Replication-coupled nucleosome assembly requires marking nascent duplexes for chaperone recruitment. Proliferating cell nuclear antigen acts as sliding clamp for Pol δ and Pol ε, encircling DNA behind polymerase. Its interdomain connector loop binds PIP motifs of chromatin assembly factor-1 subunits Cac1 and p150, concentrating CAF-1 at replication foci. CAF-1 in turn deposits newly synthesized H3.1-H4 dimers onto emerging DNA, facilitating rapid chromatin restoration. Subsequent PCNA unloading by ATAD5 is coordinated with assembly efficiency, linking fork speed to histone supply and ensuring epigenome maintenance during S phase.