Practice question

RNase P is essential ribonucleoprotein endonuclease best known for processing five prime leader removal from precursor transfer RNAs across all domains of life. Bacterial RNase P consists of catalytic M1 RNA of approximately three hundred seventy-seven nucleotides capable of cleavage alone in vitro, classic ribozyme example, plus C5 protein enhancing specificity. Eukaryotic nuclear RNase P has H1 RNA plus ten protein subunits including Pop1, Pop5, Rpp20. Reaction cleaves phosphodiester at plus one position generating mature five prime end with phosphate, leaving three prime hydroxyl of leader. Human RNase P also processes long noncoding RNAs and regulates Pol I transcription.