Practice question

lacY gene product is lactose permease LacY, 417-residue member of major facilitator superfamily transporter folded into twelve transmembrane alpha helices forming central hydrophilic cavity accessible alternately outward and inward during cycle. It operates as galactoside-proton symporter driven entirely by proton motive force, accumulating lactose against concentration gradient for metabolic use. Protein functionality is distinct from soluble beta-galactosidase encoded by lacZ and transacetylase LacA. By concentrating intracellular beta-galactosides, LacY enables efficient generation of allolactose inducer and provides substrate for catalytic cleavage, creating autocatalytic positive feedback essential for bistable switching behavior of lac operon.