Practice question

Chromatography encompasses multiple interaction mechanisms exploited across various modes. Adsorption relies on surface interactions with solid phase, partition depends on differential solubility between two liquid phases, size exclusion separates via pore accessibility, ion exchange utilizes electrostatic attraction, affinity uses biospecific recognition, and hydrophobic interaction uses polarity differences. Different analytes may be resolved by different dominant forces, but all fall under chromatographic principle of differential migration. Stating that all mechanisms are valid acknowledges versatility of technique and helps in method selection based on analyte properties such as charge, size, hydrophobicity, or specific binding capability for purification.