Practice question

Ambisense coding strategy observed in arenaviruses, phleboviruses and tospoviruses combines positive and negative polarity within same RNA segment. Each segment contains two open reading frames in opposite orientation separated by intergenic hairpin acting as transcription termination signal. One gene is expressed from genomic strand as negative-sense, the second from antigenomic complementary strand as positive-sense after replication. This arrangement allows regulation of gene expression timing and genome compactness. It differs from fully negative or positive genomes by encoding proteins from both strands of single molecule.