Practice question
Question
A YAC-derived construct must contain which sequence to survive in yeast?
Explanation
Functional yeast artificial chromosome must replicate, segregate and be selectable. Autonomously replicating sequence acts as origin where replication complex assembles, licensing DNA duplication once per cell cycle. Without ARS, linear construct fails to duplicate and is lost. Centromere ensures proper segregation, telomeres protect linear ends, but ARS provides essential replication initiation. Bacterial ori functions only in Escherichia coli for shuttling, lac operon regulates bacterial transcription, not yeast survival. Therefore presence of yeast origin ARS is indispensable for autonomous replication and maintenance of YAC construct within Saccharomyces cerevisiae nucleus.
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