Practice question
Question
Root-to-shoot conversion induced by:
Explanation
REVOLUTA belongs to class III HD-ZIP family specifying adaxial leaf fate, shoot meristem identity, and interfascicular fiber vascular patterning regulated by microRNA165/166 degradation. Ectopic constitutive 35S-driven expression of REV in root pole suppresses PLT genes, reprograms root meristem into shoot-like organization initiating leaf-like primordia expressing shoot markers STM, WUS, CLV3, and capable of producing shoots upon cytokinin treatment. Root-to-shoot conversion illustrates antagonistic relationship between shoot-promoting REV pathway and root-promoting PLT pathway, where misexpression overrides intrinsic root networks and reprograms meristem identity toward aerial fate.