Practice question

Type II restriction enzymes recognize specific palindromic hexamers and cut within them producing defined ends. Sequence 5'-GATATC-3' contains central ATAT palindrome recognized by EcoRV, which cuts between T and A on both strands generating blunt ends, unlike BamHI recognizing GGATCC or EcoRI recognizing GAATTC which produce sticky overhangs. EcoRV is frequently used to create blunt cloning junctions and for physical mapping studies. Memorizing common hexameric sites as per NCERT and typical molecular biology laboratory practice is crucial for predicting fragment ends and designing compatible cloning strategies efficiently.