Practice question

A chromatograph is the instrument comprising column, pumps, injector, and detector, while the time-dependent detector signal produced during a run forms the graphical record. This plot of signal intensity versus retention time or volume displays peaks whose position reflects interaction strength and whose area approximates quantity. Termed chromatogram, it allows calculation of resolution, plate number, and retention factor. Distinction from retention plot or generic peak chart emphasizes standardized evaluation. Interpreting chromatograms is central to qualitative and quantitative analysis in biochemistry and analytical chemistry.