Practice question

Techniques to map physical protein associations include co-immunoprecipitation that isolates native complexes via specific antibodies, fluorescence resonance energy transfer that detects proximity under 10 nm in live cells, phage display linking phenotype to genotype, yeast two-hybrid, surface plasmon resonance, and affinity pull-downs. SDS-PAGE is a fundamental analytical separation method that denatures proteins with sodium dodecyl sulfate and resolves them by molecular weight under electric field. It provides size information but alone does not report interaction without further methods like far-western or immunoblotting for detection.