Practice question
Question
Which of the following is a pull-down tag?
Explanation
Pull-down assays isolate interacting partners using affinity tags fused to bait protein. Glutathione S-transferase is a 26 kDa tag that binds with high affinity to immobilized glutathione on agarose or sepharose beads. GST-tagged bait is expressed recombinantly, captured on resin, then incubated with prey lysate. After washing, complexes are eluted using reduced glutathione. GST offers good solubility enhancement and simple purification. Unlike GFP which is fluorescent tracer and EDTA which is chelator, GST functions as both solubility and affinity handle.
Related blogs
Guides and insights connected to this topic
SARS-CoV-2 Coronavirus: Structure, Genome, Proteins, Replication, Pathogenesis, and Host Interaction
Coronavirus Overview Coronaviruses (CoVs) are enveloped viruses (surrounded by a lipid membrane derived from host cells), belonging to the s...
Coronavirus Structure, Genome, Replication, Symptoms, Pathogenesis, and Prevention
Structure of Coronavirus Coronaviruses (enveloped RNA viruses) fall in the virus family Coronaviridae, order Nidovirales. They are enveloped...
Cucumber Mosaic Virus (CMV): Structure, Genome, Symptoms, Disease Cycle and Management
Cucumber Mosaic Virus (CMV): Introduction and Importance Cucumber Mosaic Virus (CMV) (plant-infecting RNA virus) is one of the most economic...