Practice question
Question
Which of the following genes gives rise to green fluorescence?
Explanation
Green fluorescent protein, originally isolated from jellyfish Aequorea victoria, functions as an autonomous scorable marker emitting bright green fluorescence upon excitation with blue or UV light. The chromophore formed by autocatalytic cyclization of Ser65-Tyr66-Gly67 within the β-barrel requires no cofactors or substrates. Fusion of GFP to gene of interest allows real-time monitoring of gene expression, subcellular localization, and recombinant identification without cell lysis. LacZ produces blue color, luciferase produces bioluminescence via luciferin oxidation, and TetR provides antibiotic resistance rather than fluorescence.
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