Practice question
Question
What is a common tag used for affinity purification?
Explanation
Affinity purification of multiprotein complexes for interaction studies needs small, specific peptide epitopes that enable immunoprecipitation under mild conditions with minimal interference. FLAG octapeptide DYKDDDDK recognized by high-affinity anti-FLAG M2 antibody permits gentle elution with free FLAG peptide, preserving partner proteins for mass spectrometry. FITC and TRITC are fluorescent isothiocyanate dyes used for labeling, not affinity isolation. EGFP is 27 kDa fluorescent reporter useful for localization and FRET but bulky tag may sterically hinder interactions and is not optimized for resin-based purification.
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